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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using RSK2 Polyclonal antibody (23762-1-AP)

    WB analysis of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

    WB result of RSK2 antibody (23762-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSK2 transfected K-562 cells. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RSK2 Polyclonal antibody (23762-1-AP)

    WB analysis using 23762-1-AP (same clone as 23762-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using RSK2 Polyclonal antibody (23762-1-AP)

    WB analysis of MCF-7 using 23762-1-AP (same clone as 23762-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using RSK2 Polyclonal antibody (23762-1-AP)

    WB analysis of NIH/3T3 using 23762-1-AP (same clone as 23762-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using RSK2 Polyclonal antibody (23762-1-AP)

    WB analysis of HepG2 using 23762-1-AP (same clone as 23762-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using RSK2 Polyclonal antibody (23762-1-AP)

    WB analysis of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using RSK2 Polyclonal antibody (23762-1-AP)

    IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

    IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:4000) with K-562 cells lysate 3680 ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using RSK2 Polyclonal antibody (23762-1-AP)

    IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

    IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using RSK2 Polyclonal antibody (23762-1-AP)

    IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

    IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000 ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using RSK2 Polyclonal antibody (23762-1-AP)

    IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

    IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000 ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RSK2 Polyclonal antibody (23762-1-AP)

    IHC staining of human kidney using 23762-1-AP (same clone as 23762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RSK2 Polyclonal antibody (23762-1-AP)

    IHC staining of human kidney using 23762-1-AP (same clone as 23762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RSK2 Polyclonal antibody (23762-1-AP)

    IHC staining of mouse colon using 23762-1-AP (same clone as 23762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RSK2 Polyclonal antibody (23762-1-AP)

    IHC staining of mouse kidney using 23762-1-AP (same clone as 23762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using RSK2 Polyclonal antibody (23762-1-AP)

    IF Staining of mouse skeletal muscle using 23762-1-AP (same clone as 23762-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using RSK2 antibody (23762-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using RSK2 Polyclonal antibody (23762-1-AP)

    IF Staining of mouse brain using 23762-1-AP (same clone as 23762-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using RSK2 Polyclonal antibody (23762-1-AP)

    IF Staining of mouse brain using 23762-1-AP (same clone as 23762-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RSK2 Polyclonal antibody (23762-1-AP)

    IF Staining of HepG2 using 23762-1-AP (same clone as 23762-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 23762-1-AP (RSK2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using RSK2 Polyclonal antibody (23762-1-AP)

    IF Staining of HUVEC using 23762-1-AP (same clone as 23762-1-PBS)

    Immunofluorescent analysis of HUVEC cells using 23762-1-AP (RSK2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RSK2 Polyclonal antibody, PBS Only

RSK2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 23762-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

RPS6KA3, CLS, EC:2.7.11.1, HU 3, Insulin-stimulated protein kinase 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23762-1-PBS targets RSK2 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20620

Product name: Recombinant human RPS6KA3 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 666-740 aa of BC096301

Sequence: QRLTAALVLRHPWIVHWDQLPQYQLNRQDAPHLVKGAMAATYSALNRNQSPVLEPVGRSTLAQRRGIKKITSTAL

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6 kinase, 90kDa, polypeptide 3
Calculated molecular weight 740 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC096301
Gene Symbol RSK2
Gene ID (NCBI) 6197
RRIDAB_2879318
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP51812
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.

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