Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24734-1-AP targets RSBN1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20895 Product name: Recombinant human RSBN1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-411 aa of BC026155 Sequence: MAAQVGAVRVVRAVAAQEEPDKEGKEKPHAGVSPRGVKRQRRSSSGGSQEKRGRPSQEPPLAPPHRRRRSRQHPGPLPPTNAAPTVPGPVEPLLLPPPPPPSLAPAGPAVAAPLPAPSTSALFTFSPLTVSAAGPKHKGHKERHKHHHHRGPDGDPSSCGTDLKHKDKQENGERTGGVPLIKAPKRETPDENGKTQRADDFVLKKIKKKKKKKHREDMRGRRLKMYNKEVQTVCAGLTRISKEILTQGQINSTSGLNKESFRYLKDEQLCRLNLGMQEYRVPQGVQTPFMTHQEHSIRRNFLKTGTKFSNFIHEEHQSNGGALVLHAYMDELSFLSPMEMERFSEEFLALTFSENEKNAAYYALAIVHGAAAYLPDFLDYFAFNFPNTPVKMEILGKKDIETTTISNFHTQ 相同性解析による交差性が予測される生物種 |
| Full Name | round spermatid basic protein 1 |
| Calculated molecular weight | 411 aa, 46 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC026155 |
| Gene Symbol | RSBN1 |
| Gene ID (NCBI) | 54665 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VWQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RSBN1 is expressed not only in the testes, but also in the brain and ovaries. RSBN1 could play a role in both meiotic and post-meiotic progression, suggesting that it may also be involved in oogenesis. On the other hand, RSBN1 may be involved in brain development and function, as well as the regulation of neuronal cells, since another H4K20 demethylase, PHF8, regulates neuronal cell survival and brain development, while LSD1 regulates memory formation (PMID: 20622853, 26214369, 34185806).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RSBN1 antibody 24734-1-AP | Download protocol |
| WB protocol for RSBN1 antibody 24734-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |