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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RRP1B Polyclonal antibody

RRP1B Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 27723-1-AP

Synonyms

KIAA0179, Nnp1, NNP1L, RRP1, RRP1 like protein B, RRP1B

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 27723-1-AP

    WB analysis of HEK-293 using 27723-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 27723-1-AP

    WB analysis of HepG2 using 27723-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 27723-1-AP

    WB analysis of HeLa using 27723-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 27723-1-AP

    IF Staining of A431 using 27723-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 27723-1-AP (RRP1B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, K-562 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27723-1-AP targets RRP1B in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRP1B fusion protein Ag23405 相同性解析による交差性が予測される生物種
Full Name ribosomal RNA processing 1 homolog B (S. cerevisiae)
Observed molecular weight 84 kDa
GenBank accession numberBC028386
Gene symbol RRP1B
Gene ID (NCBI) 23076
RRIDAB_2880953
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RRP1B antibody 27723-1-APDownload protocol
IF protocol for RRP1B antibody 27723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols