Validation Data Gallery

Filter: AllWBIP

WB analysis of HeLa using 25918-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 25918-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 25918-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 25918-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 25918-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25918-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 25918-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 25918-1-AP
IP result of anti-RRN3 (IP:25918-1-AP, 4ug; Detection:25918-1-AP 1:500) with HeLa cells lysate 3200ug.

RRN3 Polyclonal antibody

RRN3 Polyclonal Antibody for WB, IP, ELISA

Cat No. 25918-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, chIP, ELISA

Synonyms

RNA polymerase I-specific transcription initiation factor RRN3, TIF IA, TIFIA, TIF-IA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, K-562 cells, mouse testis tissue
Positive IP detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
ChIP	See 1 publications below

Product Information

25918-1-AP targets RRN3 in WB, IP, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag23207 Product name: Recombinant human RRN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 268-365 aa of BC132688 Sequence: ETATQTCGGTDSTEGLFNMDEDEETEHETKAGPERLDQMVHPVAERLDILMSLVLSYMKDVCYVDGKVDNGKTKDLYRDLINIFDKLLLPTHASCHVQ 相同性解析による交差性が予測される生物種
Full Name	RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
Observed molecular weight	74 kDa
GenBank accession number	BC132688
Gene Symbol	RRN3
Gene ID (NCBI)	54700
RRID	AB_2880296
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NYV6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IP protocol for RRN3 antibody 25918-1-AP	Download protocol
WB protocol for RRN3 antibody 25918-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	chIP	Biochem Biophys Res Commun Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression. Authors - Xiaolei Zhang View Article
	WB	Mol Cell Control of ribosomal RNA synthesis by hematopoietic transcription factors Authors - Charles Antony View Article
human	WB	Mol Biol Cell A role for the kinetochore protein, NUF2, in ribosome biogenesis Authors - Ty J Brown View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 25918-1-AP

WB analysis using 25918-1-AP

WB analysis of K-562 using 25918-1-AP

WB analysis of Jurkat using 25918-1-AP

WB analysis of mouse testis using 25918-1-AP