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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RRM2B,p53R2 Polyclonal antibody (18005-1-AP)

    WB analysis using 18005-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using RRM2B,p53R2 Polyclonal antibody (18005-1-AP)

    WB analysis of MDA-MB-453s using 18005-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RRM2B,p53R2 Polyclonal antibody (18005-1-AP)

    WB analysis of HeLa using 18005-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RRM2B,p53R2 Polyclonal antibody (18005-1-AP)

    WB analysis of PC-3 using 18005-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RRM2B,p53R2 Polyclonal antibody (18005-1-AP)

    WB analysis of PC-3 using 18005-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RRM2B,p53R2 Polyclonal antibody (18005-1-AP)

    IHC staining of human stomach using 18005-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

RRM2B,p53R2 Polyclonal antibody

RRM2B,p53R2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18005-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

RRM2B, TP53-inducible ribonucleotide reductase M2 B, Ribonucleoside-diphosphate reductase subunit M2 B, p53R2, p53-inducible ribonucleotide reductase small subunit 2-like protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, PC-3 cells, MDA-MB-453s cells, HeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18005-1-AP targets RRM2B,p53R2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRM2B,p53R2 fusion protein Ag12499 相同性解析による交差性が予測される生物種
Full Name ribonucleotide reductase M2 B (TP53 inducible)
Calculated molecular weight 351 aa, 41 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC108261
Gene Symbol RRM2B
Gene ID (NCBI) 50484
RRIDAB_2878480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7LG56
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RRM2B,p53R2 antibody 18005-1-APDownload protocol
IHC protocol for RRM2B,p53R2 antibody 18005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Ther

DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.

Authors - Fangfang Zhang
  • KD Validated
View Article