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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using RRM1 Monoclonal antibody (60073-2-Ig)

    WB analysis of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60073-2-Ig (RRM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RRM1 Monoclonal antibody (60073-2-Ig)

    WB analysis of HeLa using 60073-2-Ig (same clone as 60073-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60073-2-Ig (RRM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using RRM1 Monoclonal antibody (60073-2-Ig)

    WB analysis of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60073-2-Ig (RRM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using RRM1 Monoclonal antibody (60073-2-Ig)

    IP experiment of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    IP result of anti-RRM1 (IP:60073-2-Ig, 5ug; Detection:60073-2-Ig 1:1000) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human pancreas cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human lung cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human lung cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human urothelial carcinoma using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human urothelial carcinoma using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human colon cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human colon cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human pancreas cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human pancreas cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IF Staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RRM1 antibody (60073-2-Ig, Clone: 5H6F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RRM1 Monoclonal antibody (60073-2-Ig)

    IF Staining of HepG2 using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RRM1 antibody (60073-2-Ig, Clone: 5H6F3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RRM1 Monoclonal antibody (60073-2-Ig)

    IF Staining of HeLa using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunofluorescent analysis of HeLa cells using 60073-2-Ig (RRM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RRM1 Monoclonal antibody, PBS Only

RRM1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 60073-2-PBS
Clone No.5H6F3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit, EC:1.17.4.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60073-2-PBS targets RRM1 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0789

Product name: Recombinant human RRM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 593-792 aa of BC006498

Sequence: IRNSLLIAPMPTASTAQILGNNESIEPYTSNIYTRRVLSGEFQIVNPHLLKDLTERGLWHEEMKNQIIACNGSIQSIPEIPDDLKQLYKTVWEISQKTVLKMAAERGAFIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTGMYYLRTRPAANPIQFTLNKEKLKDKEKVSKEEEEKERNTAAMVCSLENRDECLMCGS

相同性解析による交差性が予測される生物種
Full Name ribonucleotide reductase M1
Calculated molecular weight 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC006498
Gene Symbol RRM1
Gene ID (NCBI) 6240
RRIDAB_10597538
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP23921
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).