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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RRBP1 Recombinant monoclonal antibody (82890-1-RR)

    IF Staining of HeLa using 82890-1-RR (same clone as 82890-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RRBP1 antibody (82890-1-RR, Clone: 230159G6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 82890-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RRBP1 Recombinant monoclonal antibody (82890-1-RR)

    IF Staining of NIH/3T3 using 82890-1-RR (same clone as 82890-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RRBP1 antibody (82890-1-RR, Clone: 230159G6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82890-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RRBP1 Recombinant monoclonal antibody (82890-1-RR)

    IF Staining of NIH/3T3 using 82890-1-RR (same clone as 82890-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RRBP1 antibody (82890-1-RR, Clone: 230159G6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 82890-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-2 OS using RRBP1 Recombinant monoclonal antibody (82890-1-RR)

    FC experiment of U-2 OS using 82890-1-RR (same clone as 82890-1-PBS)

    (blue)1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human RRBP1 (82890-1-RR, Clone:230159G6) and Alexa Fluor™ Plus 647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82890-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RRBP1 Recombinant monoclonal antibody (82890-1-RR)

    FC experiment of HeLa using 82890-1-RR (same clone as 82890-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RRBP1 Recombinant antibody (82890-1-RR, Clone:230159G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82890-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82890-1-RR

    Affinity and Kinetic Characterization of 82890-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82890-1-RR against Human RRBP1 were performed. The affinity constant is below 1 pM.

RRBP1 Recombinant monoclonal antibody, PBS Only

RRBP1 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82890-1-PBS
Clone No.230159G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

180 kDa ribosome receptor homolog, 230159G6, ES/130 related protein, ES/130-related protein, Ribosome-binding protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82890-1-PBS targets RRBP1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag16648

Product name: Recombinant human RRBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1061-1410 aa of BC128577

Sequence: AQTMEALLALLPELSVLAQQNYTEWLQDLKEKGPTLLKHPPAPAEPSSDLASKLREAEETQSTLQAECDQYRSILAETEGMLRDLQKSVEEEEQVWRAKVGAAEEELQKSRVTVKHLEEIVEKLKGELESSDQVREHTLHLEAELEKHMAAASAECQNYAKEVAGLRQLLLESQSQLDAAKSEAQKQSDELALVRQQLSEMKSHVEDGDIAGAPASSPEAPPAEQDPVQLKTQLEWTEAILEDEQTQRQKLTAEFEEAQTSACRLQEELEKLRTAGPLESSETEEASQLKERLEKEKKLTSDLGRAATRLQELLKTTQEQLAREKDTVKKLQEQLEKAEDGSSSKEGTSV

相同性解析による交差性が予測される生物種
Full Name ribosome binding protein 1 homolog 180kDa (dog)
Calculated molecular weight 1410 aa, 152 kDa
Observed molecular weight108 kDa, 152 kDa
GenBank accession numberBC128577
Gene Symbol RRBP1
Gene ID (NCBI) 6238
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9P2E9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)

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