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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using RRAS Monoclonal antibody (66959-1-Ig)

    WB analysis of A549 using 66959-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66959-1-Ig (RRAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RRAS Monoclonal antibody (66959-1-Ig)

    WB analysis of PC-3 using 66959-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66959-1-Ig (RRAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using RRAS Monoclonal antibody (66959-1-Ig)

    WB analysis of HT-29 using 66959-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66959-1-Ig (RRAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using RRAS Monoclonal antibody (66959-1-Ig)

    WB analysis of HSC-T6 using 66959-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66959-1-Ig (RRAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RRAS Monoclonal antibody (66959-1-Ig)

    WB analysis of NIH/3T3 using 66959-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66959-1-Ig (RRAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

RRAS Monoclonal antibody

RRAS Monoclonal Antibody for WB, ELISA
Cat No. 66959-1-Ig
Clone No.2E1A12

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, Rat

Applications

WB, ELISA

Synonyms

p23, Ras related protein R Ras, RRAS

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, PC-3 cells, HT-29 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:4000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66959-1-Ig targets RRAS in WB, ELISA applications and shows reactivity with Human, mouse, Rat samples.

Tested Reactivity Human, mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26564

Product name: Recombinant human RRAS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 145-218 aa of BC016318

Sequence: DLESQRQVPRSEASAFGASHHVAYFEASAKLRLNVDEAFEQLVRAVRKYQEQELPPSPPSAPRKKGGGCPCVLL

相同性解析による交差性が予測される生物種
Full Name related RAS viral (r-ras) oncogene homolog
Calculated molecular weight 218 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC016318
Gene Symbol RRAS
Gene ID (NCBI) 6237
RRIDAB_2882282
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10301
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RRAS belongs to the Ras subfamily of small G-proteins, which are frequently implicated in cell growth and differentiation.RRAS is a small GTPase involved in diverse processes including angiogenesis, vascular homeostasis and regeneration, cell adhesion, and neuronal axon guidance. Mutations in this gene are found in many invasive cancers.

Protocols

Product Specific Protocols
WB protocol for RRAS antibody 66959-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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