Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | NIH/3T3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15315-1-AP targets RRAGD in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7557 Product name: Recombinant human RRAGD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-249 aa of BC003088 Sequence: MEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTPVDMQTYELCCDMIDVVIDISCIYGLKEDGAGTPYDKESTAIIKLNNTTVLYLKEVTKFLALVCFVREESFERKGLIDYNFHCFRKAIHEVFEVRMKVVKSRKVQNRLQKKKRATPNGTPRVLL 相同性解析による交差性が予測される生物種 |
| Full Name | Ras-related GTP binding D |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 46-50 kDa |
| GenBank accession number | BC003088 |
| Gene Symbol | RRAGD |
| Gene ID (NCBI) | 58528 |
| RRID | AB_3085458 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQL2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ras-related GTP-binding protein D (RRAGD) is a monomeric GTP or GDP binding protein, which plays a critical role in mediating amino acid stimulated mTOR signaling pathway, a key pathway in determination of cell growth and proliferation rate. Recently, RRAGD has been reported involving the progression of ovarian cancer and colon cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RRAGD antibody 15315-1-AP | Download protocol |
| IP protocol for RRAGD antibody 15315-1-AP | Download protocol |
| WB protocol for RRAGD antibody 15315-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |