• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RRAGD Polyclonal antibody

RRAGD Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15315-1-AP

Synonyms

Rag D, RAGD, Ras related GTP binding D, RRAGD

Validation Data Gallery

Applications:
  • WB analysis using 15315-1-AP

    WB analysis using 15315-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15315-1-AP (RRAGD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of NIH/3T3 using 15315-1-AP

    IP experiment of NIH/3T3 using 15315-1-AP

    IP result of anti-RRAGD (IP:15315-1-AP, 4ug; Detection:15315-1-AP 1:500) with NIH/3T3 cells lysate 1120 ug.

Tested Applications

Positive WB detected inNIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

15315-1-AP targets RRAGD in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRAGD fusion protein Ag7557 相同性解析による交差性が予測される生物種
Full Name Ras-related GTP binding D
Calculated molecular weight 46 kDa
Observed molecular weight46-50 kDa
GenBank accession numberBC003088
Gene symbol RRAGD
Gene ID (NCBI) 58528
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ras-related GTP-binding protein D (RRAGD) is a monomeric GTP or GDP binding protein, which plays a critical role in mediating amino acid stimulated mTOR signaling pathway, a key pathway in determination of cell growth and proliferation rate. Recently, RRAGD has been reported involving the progression of ovarian cancer and colon cancer.

Protocols

Product Specific Protocols
WB protocol for RRAGD antibody 15315-1-APDownload protocol
IP protocol for RRAGD antibody 15315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols