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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPUSD1 Polyclonal antibody (25578-1-AP)

    WB analysis using 25578-1-AP (same clone as 25578-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25578-1-AP (RPUSD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25578-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RPUSD1 Polyclonal antibody (25578-1-AP)

    IP experiment of HeLa using 25578-1-AP (same clone as 25578-1-PBS)

    IP result of anti-RPUSD1 (IP:25578-1-AP, 4ug; Detection:25578-1-AP 1:500) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 25578-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPUSD1 Polyclonal antibody (25578-1-AP)

    IF Staining of HeLa using 25578-1-AP (same clone as 25578-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPUSD1 antibody (25578-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25578-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25578-1-PBS targets RPUSD1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPUSD1 fusion protein Ag22314 相同性解析による交差性が予測される生物種
Full Name RNA pseudouridylate synthase domain containing 1
Calculated molecular weight 312 aa, 35 kDa
Observed molecular weight34~35 kDa
GenBank accession numberBC011783
Gene Symbol RPUSD1
Gene ID (NCBI) 113000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJJ7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RPUSD1 (RNA pseudouridylate synthase domain-containing protein 1) also known as RLUCL (Ribosomal large subunit pseudouridine synthase C-like protein), was significantly upregulated in HCC and expressed at higher levels in advanced stage or poorly differentiated HCC tissues (PMID: 36437949).