Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of mouse uterus using 18215-1-AP
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 18215-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 18215-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18215-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human lung using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 18215-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 18215-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPS9 antibody (18215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

RPS9 Polyclonal antibody

RPS9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18215-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

40S ribosomal protein S9, ribosomal protein S9, Small ribosomal subunit protein uS4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (11)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse uterus tissue, HeLa cells, mouse ovary tissue
Positive IHC detected in	human lung tissue, human ovary tissue, human skin tissue, human testis tissue, mouse lung tissue, mouse pancreas tissue, mouse stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 8 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

18215-1-AP targets RPS9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12945 Product name: Recombinant human RPS9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-194 aa of BC000802 Sequence: MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRRLVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEED 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein S9
Calculated molecular weight	23 kDa
Observed molecular weight	25 kDa
GenBank accession number	BC000802
Gene Symbol	RPS9
Gene ID (NCBI)	6203
RRID	AB_2182390
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P46781
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for RPS9 antibody 18215-1-AP	Download protocol
IHC protocol for RPS9 antibody 18215-1-AP	Download protocol
WB protocol for RPS9 antibody 18215-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Mol Psychiatry Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome Authors - Federica Giona View Article
mouse	WB	J Transl Med Paternal ribosome biogenesis dysfunction caused by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice. Authors - Xin Li View Article
human	WB	Proc Natl Acad Sci U S A Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein. Authors - Elizabeth I Vink KD Validated View Article
human	IHC	Proc Natl Acad Sci U S A Local translation in nuclear condensate amyloid bodies. Authors - Phaedra R Theodoridis View Article
mouse	WB	Dev Cell Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility. Authors - Yuqing Lei View Article
human	IF	Cell Rep Senescent cells form nuclear foci that contain the 26S proteasome Authors - Tomohiro Iriki View Article

Validation Data Gallery

WB analysis of mouse uterus using 18215-1-AP

WB analysis of mouse ovary using 18215-1-AP

WB analysis of mouse ovary using 18215-1-AP

WB analysis of HeLa using 18215-1-AP

IHC staining of human lung using 18215-1-AP

IHC staining of human lung using 18215-1-AP

IHC staining of mouse lung using 18215-1-AP

IHC staining of mouse pancreas using 18215-1-AP

IHC staining of mouse stomach using 18215-1-AP

IHC staining of human testis using 18215-1-AP

IHC staining of human testis using 18215-1-AP

IHC staining of human skin using 18215-1-AP

IHC staining of human skin using 18215-1-AP

IHC staining of human lung using 18215-1-AP

IHC staining of human lung using 18215-1-AP

IHC staining of human ovary using 18215-1-AP

IHC staining of human ovary using 18215-1-AP

IF Staining of HeLa using 18215-1-AP

RPS9 Polyclonal antibody

RPS9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome

Paternal ribosome biogenesis dysfunction caused by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice.

Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.

Local translation in nuclear condensate amyloid bodies.

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Senescent cells form nuclear foci that contain the 26S proteasome

Able™

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