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Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of DU 145 using 17296-1-AP (same clone as 17296-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A2780 cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of A2780 using 17296-1-AP (same clone as 17296-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SKOV-3 cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of SKOV-3 using 17296-1-AP (same clone as 17296-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of mouse testis using 17296-1-AP (same clone as 17296-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of DU 145 using 17296-1-AP (same clone as 17296-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A2780 cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of A2780 using 17296-1-AP (same clone as 17296-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of mouse ovary using 17296-1-AP (same clone as 17296-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of HeLa using 17296-1-AP (same clone as 17296-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using RPS4Y1 Polyclonal antibody (17296-1-AP)

    IHC staining of human appendicitis using 17296-1-AP (same clone as 17296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17296-1-AP (RPS4Y1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    IF Staining of HepG2 using 17296-1-AP (same clone as 17296-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS4Y1 antibody (17296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

RPS4Y1 Polyclonal antibody, PBS Only

RPS4Y1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17296-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Small ribosomal subunit protein eS4, Y isoform 1, RPS4Y, PRO2646, 40S ribosomal protein S4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17296-1-PBS targets RPS4Y1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11176

Product name: Recombinant human RPS4Y1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 6-263 aa of BC010286

Sequence: KKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIVFLRNRLKYALTGDEVKKICMQRFIKIDGKVRVDVTYPAGFMDVISIEKTGEHFRLVYDTKGRFAVHRITVEEAKYKLCKVRKITVGVKGIPHLVTHDARTIRYPDPVIKVNDTVQIDLGTGKIINFIKFDTGNLCMVIGGANLGRVGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGNGNKPWISLPRGKGIRLTVAEERDKRLATKQSSG

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S4, Y-linked 1
Calculated molecular weight 263 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC010286
Gene Symbol RPS4Y1
Gene ID (NCBI) 6192
RRIDAB_2180889
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP22090
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RPS4Y1, a Y-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). RPS4Y maps to a 90-kb segment that has been implicated in Turner syndrome. As a homolog with RPS4X, the antibody can recognize endogenous level of RPS4Y and RPS4X, at 29-32kDa.

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