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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS3A Polyclonal antibody (14123-1-AP)

    WB analysis using 14123-1-AP (same clone as 14123-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using RPS3A Polyclonal antibody (14123-1-AP)

    WB analysis of A431 using 14123-1-AP (same clone as 14123-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using RPS3A Polyclonal antibody (14123-1-AP)

    WB analysis of Jurkat using 14123-1-AP (same clone as 14123-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using RPS3A Polyclonal antibody (14123-1-AP)

    WB analysis of A549 using 14123-1-AP (same clone as 14123-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RPS3A Polyclonal antibody (14123-1-AP)

    IP experiment of HeLa using 14123-1-AP (same clone as 14123-1-PBS)

    IP result of anti-RPS3A (IP:14123-1-AP, 4ug; Detection:14123-1-AP 1:6000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using RPS3A Polyclonal antibody (14123-1-AP)

    IHC staining of human colon using 14123-1-AP (same clone as 14123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14123-1-AP (RPS3A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS3A Polyclonal antibody (14123-1-AP)

    IF Staining of HepG2 using 14123-1-AP (same clone as 14123-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS3A antibody (14123-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using RPS3A Polyclonal antibody (14123-1-AP)

    FC experiment of HepG2 using 14123-1-AP (same clone as 14123-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human RPS3A (14123-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RPS3A Polyclonal antibody, PBS Only

RPS3A Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 14123-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

40S ribosomal protein S3a, Fte 1, FTE1, Fte-1, MFTL

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14123-1-PBS targets RPS3A in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5273

Product name: Recombinant human RPS3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-264 aa of BC066926

Sequence: MAVSKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVAFRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQVRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGKATGDETGAKVERADGYEPPVQESV

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S3A
Calculated molecular weight 30 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC066926
Gene Symbol RPS3A
Gene ID (NCBI) 6189
RRIDAB_2253921
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP61247
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors.

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