Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse testis tissue, PC-12 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 18 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
Product Information
11990-1-AP targets RPS3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3918 Product name: Recombinant human RPS3 protein Source: e coli.-derived, Unknow Tag: N/A Domain: c16032 of BC034149 Sequence: 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S3 |
| Calculated molecular weight | 26 aa, 7 kDa |
| Observed molecular weight | 26.7 kDa |
| GenBank accession number | BC034149 |
| Gene Symbol | RPS3 |
| Gene ID (NCBI) | 6188 |
| RRID | AB_2180758 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23396 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS3 antibody 11990-1-AP | Download protocol |
| IHC protocol for RPS3 antibody 11990-1-AP | Download protocol |
| IP protocol for RPS3 antibody 11990-1-AP | Download protocol |
| WB protocol for RPS3 antibody 11990-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs | ||
Mol Cell A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation | ||
Nat Commun Cilia locally synthesize proteins to sustain their ultrastructure and functions. | ||
Curr Biol RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. | ||
J Anim Sci Biotechnol Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs. | ||
Front Oncol RPS3 Promotes the Metastasis and Cisplatin Resistance of Adenoid Cystic Carcinoma. |
