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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS29 Polyclonal antibody

RPS29 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17374-1-AP

Synonyms

40S ribosomal protein S29, ribosomal protein S29, RPS29

Validation Data Gallery

Applications:
  • WB analysis using 17374-1-AP

    WB analysis using 17374-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17374-1-AP (RPS29 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 17374-1-AP

    IHC staining of mouse brain using 17374-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17374-1-AP (RPS29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17374-1-AP

    IHC staining of mouse brain using 17374-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17374-1-AP (RPS29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17374-1-AP targets RPS29 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS29 fusion protein Ag11179 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S29
Calculated molecular weight 56 aa, 7 kDa
Observed molecular weight 7 kDa
GenBank accession numberBC035313
Gene symbol RPS29
Gene ID (NCBI) 6235
RRIDAB_2180751
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPS29 antibody 17374-1-APDownload protocol
IHC protocol for RPS29 antibody 17374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols