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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS23 Polyclonal antibody (29834-1-AP)

    WB analysis using 29834-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29834-1-AP (RPS23 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RPS23 Polyclonal antibody (29834-1-AP)

    IF Staining of MCF-7 using 29834-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS23 antibody (29834-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RPS23 Polyclonal antibody

RPS23 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29834-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, U2OS cells, human placenta tissue, mouse ovary tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29834-1-AP targets RPS23 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS23 fusion protein Ag31190 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S23
Calculated molecular weight 16 kDa
Observed molecular weight 16-18 kDa
GenBank accession numberNM_001025
Gene Symbol RPS23
Gene ID (NCBI) 6228
RRIDAB_3669698
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62266
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS23 (40S ribosomal protein S23), also called small ribosomal subunit protein uS12, is a subunit of the 40S ribosome and the first precursor of the small eukaryotic ribosomal subunit (PMID: 34516797). It is positioned in the decoding center of the ribosome that serves to maintain translational fidelity by monitoring the complementarity between the mRNA codons being translated and the anti-codons of aminoacyl-tRNAs (PMID: 28257692; 23636399).

Protocols

Product Specific Protocols
WB protocol for RPS23 antibody 29834-1-APDownload protocol
IF protocol for RPS23 antibody 29834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols