Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 16946-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 16946-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16946-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of SGC-7901 using 16946-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 16946-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16946-1-AP (RPS21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16946-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16946-1-AP (RPS21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16946-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS21 antibody (16946-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Featured Product
KD/KO Validated

RPS21 Polyclonal antibody

RPS21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16946-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Small ribosomal subunit protein eS21, ribosomal protein S21, 40S ribosomal protein S21

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, mouse liver tissue, SGC-7901 cells, rat liver tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 3 publications below

Product Information

16946-1-AP targets RPS21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10557 Product name: Recombinant human RPS21 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-81 aa of BC018140 Sequence: MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVSK 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein S21
Calculated molecular weight	81 aa, 9 kDa
Observed molecular weight	9 kDa
GenBank accession number	BC018140
Gene Symbol	RPS21
Gene ID (NCBI)	6227
RRID	AB_2180351
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P63220
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit.

Protocols

Product Specific Protocols
IF protocol for RPS21 antibody 16946-1-AP	Download protocol
IHC protocol for RPS21 antibody 16946-1-AP	Download protocol
WB protocol for RPS21 antibody 16946-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Endocrinology Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice Authors - Abraham Behrmann View Article
		J Cell Sci Drp1 regulates transcription of ribosomal protein genes in embryonic hearts. Authors - Qiancong Zhao View Article
human	IHC	Oncol Lett Identification of the key genes and pathways in prostate cancer. Authors - Shutong Fan View Article
human	WB,IHC	Med Oncol Identification of candidate diagnostic and prognostic biomarkers for human prostate cancer: RPL22L1 and RPS21. Authors - Zumu Liang KD Validated View Article
mouse	IHC	Reprod Sci Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes Authors - Natsuki Nakanishi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16946-1-AP

WB analysis using 16946-1-AP

WB analysis of HepG2 using 16946-1-AP

WB analysis of SGC-7901 using 16946-1-AP

IHC staining of human liver cancer using 16946-1-AP

IHC staining of human liver cancer using 16946-1-AP

IF Staining of HepG2 using 16946-1-AP

RPS21 Polyclonal antibody

RPS21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice

Drp1 regulates transcription of ribosomal protein genes in embryonic hearts.

Identification of the key genes and pathways in prostate cancer.

Identification of candidate diagnostic and prognostic biomarkers for human prostate cancer: RPL22L1 and RPS21.

Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes