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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS18 Recombinant monoclonal antibody (83327-1-RR)

    IF Staining of HepG2 using 83327-1-RR (same clone as 83327-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS18 antibody (83327-1-RR, Clone: 240004C10 ) at dilution of 1:0 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83327-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using RPS18 Recombinant monoclonal antibody (83327-1-RR)

    FC experiment of HepG2 using 83327-1-RR (same clone as 83327-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RPS18 Recombinant antibody (83327-1-RR, Clone:240004C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83327-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83327-1-RR

    Affinity and Kinetic Characterization of 83327-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83327-1-RR against Human RPS18 were performed. The affinity constant is 0.47 nM.

RPS18 Recombinant monoclonal antibody, PBS Only

RPS18 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83327-1-PBS
Clone No.240004C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

240004C10, D6S218E, HKE3, KE 3, KE3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83327-1-PBS targets RPS18 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag12505

Product name: Recombinant human RPS18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-152 aa of BC106063

Sequence: MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S18
Calculated molecular weight 152 aa, 18 kDa
GenBank accession numberBC106063
Gene Symbol RPS18
Gene ID (NCBI) 6222
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP62269
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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