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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS14 Monoclonal antibody (67566-1-Ig)

    WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RPS14 Monoclonal antibody (67566-1-Ig)

    WB analysis of HeLa using 67566-1-Ig (same clone as 67566-1-PBS)

    WB result of RPS14 antibody (67566-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS14 transfected HeLa cells. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RPS14 Monoclonal antibody (67566-1-Ig)

    WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RPS14 Monoclonal antibody (67566-1-Ig)

    WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using RPS14 Monoclonal antibody (67566-1-Ig)

    IHC staining of human urothelial carcinoma using 67566-1-Ig (same clone as 67566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67566-1-Ig (RPS14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using RPS14 Monoclonal antibody (67566-1-Ig)

    IHC staining of human urothelial carcinoma using 67566-1-Ig (same clone as 67566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67566-1-Ig (RPS14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS14 Monoclonal antibody (67566-1-Ig)

    IF Staining of HepG2 using 67566-1-Ig (same clone as 67566-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS14 antibody (67566-1-Ig, Clone: 3D5H5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RPS14 Monoclonal antibody, PBS Only

RPS14 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67566-1-PBS
Clone No.3D5H5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RPS 14, ribosomal protein S14, PRO2640, EMTB, 40S ribosomal protein S14

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67566-1-PBS targets RPS14 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30032

Product name: Recombinant human RPS14 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-151 aa of BC003401

Sequence: MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S14
Calculated molecular weight 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC003401
Gene Symbol RPS14
Gene ID (NCBI) 6208
RRIDAB_2882780
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP62263
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].

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