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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS12 Monoclonal antibody (67683-1-Ig)

    WB analysis using 67683-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RPS12 Monoclonal antibody (67683-1-Ig)

    WB analysis of HepG2 using 67683-1-Ig

    WB result of RPS12 antibody (67683-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS12 transfected HepG2 cells.

  • Western Blot (WB) analysis of NCI-H1299 cells using RPS12 Monoclonal antibody (67683-1-Ig)

    WB analysis of NCI-H1299 using 67683-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPS12 Monoclonal antibody (67683-1-Ig)

    IHC staining of human breast cancer using 67683-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS12 Monoclonal antibody (67683-1-Ig)

    IHC staining of human colon cancer using 67683-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS12 Monoclonal antibody (67683-1-Ig)

    IHC staining of human colon cancer using 67683-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPS12 Monoclonal antibody (67683-1-Ig)

    IHC staining of human breast cancer using 67683-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS12 Monoclonal antibody (67683-1-Ig)

    IF Staining of HepG2 using 67683-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS12 antibody (67683-1-Ig, Clone: 1A3D1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS12 Monoclonal antibody (67683-1-Ig)

    IF Staining of HepG2 using 67683-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS12 antibody (67683-1-Ig, Clone: 1A3D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Featured Product
  • KD/KO Validated

RPS12 Monoclonal antibody

RPS12 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67683-1-Ig
Clone No.1A3D1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1A3D1, 40S ribosomal protein S12, ribosomal protein S12, Small ribosomal subunit protein eS12

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NCI-H1299 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, PC-12cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67683-1-Ig targets RPS12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9649

Product name: Recombinant human RPS12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-132 aa of BC017321

Sequence: MAEEGIAAGGVMDVNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVLASNCDEPMYVKLVEALCAEHQINLIKVDDNKKLGEWVGLCKIDREGKPRKVVGCSCVVVKDYGKESQAKDVIEEYFKCKK

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S12
Calculated molecular weight 132 aa, 15 kDa
Observed molecular weight15 kDa
GenBank accession numberBC017321
Gene Symbol RPS12
Gene ID (NCBI) 6206
RRIDAB_2882876
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP25398
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.

Protocols

Product Specific Protocols
IF protocol for RPS12 antibody 67683-1-IgDownload protocol
IHC protocol for RPS12 antibody 67683-1-IgDownload protocol
WB protocol for RPS12 antibody 67683-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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