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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS11 Polyclonal antibody (15942-1-AP)

    WB analysis using 15942-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15942-1-AP (RPS11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RPS11 Polyclonal antibody (15942-1-AP)

    IHC staining of human stomach cancer using 15942-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15942-1-AP (RPS11 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RPS11 Polyclonal antibody

RPS11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15942-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Small ribosomal subunit protein uS17, ribosomal protein S11, 40S ribosomal protein S11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, MDA-MB-453s cells, ROS1728 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15942-1-AP targets RPS11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8346

Product name: Recombinant human RPS11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-158 aa of BC007945

Sequence: MADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGVVTKMKMQRTIVIRRDYLHYIRKYNRFEKRHKNMSVHLSPCFRDVQIGDIVTVGECRPLSKTVRFNVLKVTKAAGTKKQFQKF

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S11
Calculated molecular weight 158 aa, 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC007945
Gene Symbol RPS11
Gene ID (NCBI) 6205
RRIDAB_3085476
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP62280
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS11 is a ribosomal protein involved in ribosome biogenesis. It is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

Protocols

Product Specific Protocols
WB protocol for RPS11 antibody 15942-1-APDownload protocol
IHC protocol for RPS11 antibody 15942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols