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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS10 Monoclonal antibody (67609-1-Ig)

    WB analysis using 67609-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67609-1-Ig (RPS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

RPS10 Monoclonal antibody

RPS10 Monoclonal Antibody for WB, ELISA
Cat No. 67609-1-Ig
Clone No.1G1E1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

1G1E1, ribosomal protein S10, Small ribosomal subunit protein eS10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67609-1-Ig targets RPS10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7018

Product name: Recombinant human RPS10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-165 aa of BC001032

Sequence: MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S10
Calculated molecular weight 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC001032
Gene Symbol RPS10
Gene ID (NCBI) 6204
RRIDAB_2882815
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP46783
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS10 (ribosomal protein S10) encodes a ribosomal protein that is a component of the small 40S subunit of the ribosome. RPS10 is essential for cell proliferation and methylation. In nucleoli, RPS10 interacts with nucleophosmin/B23 and is predominantly concentrated in the granular component region, which is required for ribosome assembly (PMID: 20159986, 23723321). Human RPS10 protein consists of 165 amino acids, with a molecular weight of approximately 18.5 kDa.

Protocols

Product Specific Protocols
WB protocol for RPS10 antibody 67609-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols