Validation Data Gallery
Tested Applications
| Positive WB detected in | A-204 cells, A431 cells, BxPC-3 cells, DU 145 cells, Raji cells, Ramos cells, THP-1 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33447-1-AP targets RPP25L in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37795 Product name: Recombinant human C9orf23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-163 aa of BC032136 Sequence: MEHYRKAGSVELPAPSPMPQLPPDTLEMRVRDGSKIRNLLGLALGRLEGGSARHVVFSGSGRAAGKAVSCAEIVKRRVPGLHQLTKLRFLQTEDSWVPASPDTGLDPLTVRRHVPAVWVLLSRDPLDPNECGYQPPGAPPGLGSMPSSSCGPRSRRRARDTRS 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 9 open reading frame 23 |
| Observed molecular weight | 17-19 kDa |
| GenBank accession number | BC032136 |
| Gene Symbol | C9orf23 |
| Gene ID (NCBI) | 138716 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8N5L8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPP25L (Ribonuclease P protein subunit p25-like protein), also known as C9orf23. RPP25L is a subunit of RNase P complex, which is mainly involved in the processing of tRNA precursor, and is responsible for cutting off the 5' leading sequence of tRNA precursor to generate mature tRNA. In addition, RPP25L may also participate in other RNA metabolism processes, such as the processing of rRNA.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPP25L antibody 33447-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |