×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using Ribophorin I Recombinant antibody (83250-2-RR)

    WB analysis of HepG2 using 83250-2-RR

    WB result of Ribophorin I antibody (83250-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribophorin I transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using RPN1 Recombinant antibody (83250-2-RR)

    WB analysis using 83250-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83250-2-RR (RPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Ribophorin I Recombinant antibody (83250-2-RR)

    IHC staining of human stomach cancer using 83250-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Ribophorin I Recombinant monoclonal antibody (83250-2-RR)

    IHC staining of human placenta using 83250-2-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Ribophorin I Recombinant monoclonal antibody (83250-2-RR)

    IHC staining of human thyroid cancer using 83250-2-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse adrenal gland tissue using Ribophorin I Recombinant monoclonal antibody (83250-2-RR)

    IHC staining of mouse adrenal gland using 83250-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using Ribophorin I Recombinant monoclonal antibody (83250-2-RR)

    IHC staining of mouse stomach using 83250-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using Ribophorin I Recombinant monoclonal antibody (83250-2-RR)

    IHC staining of rat kidney using 83250-2-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using Ribophorin I Recombinant monoclonal antibody (83250-2-RR)

    IHC staining of rat stomach using 83250-2-RR

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using RPN1 Recombinant antibody (83250-2-RR)

    FC experiment of HeLa using 83250-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPN1 Recombinant antibody (83250-2-RR, Clone:240044D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83250-2-RR

    Affinity and Kinetic Characterization of 83250-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83250-2-RR against Human RPN1 were performed. The affinity constant is 0.154 uM.

  • Featured Product
  • KD/KO Validated

Ribophorin I Recombinant monoclonal antibody

Ribophorin I Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83250-2-RR
Clone No.240044D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

RPN1, 240044D4, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Ribophorin-1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HepG2 cells, HeLa cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue, mouse stomach tissue, human thyroid cancer tissue, mouse adrenal gland tissue, rat stomach tissue, human placenta tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83250-2-RR targets Ribophorin I in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3576

Product name: Recombinant human RPN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-350 aa of BC010839

Sequence: GSASSEAPPLINEDVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSFKTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGYNLPSYEYLYNLGDQYA

相同性解析による交差性が予測される生物種
Full Name ribophorin I
Calculated molecular weight 69 kDa
Observed molecular weight 66-69 kDa
GenBank accession numberBC010839
Gene Symbol RPN1
Gene ID (NCBI) 6184
RRIDAB_3670925
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP04843
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for Ribophorin I antibody 83250-2-RRDownload protocol
IHC protocol for Ribophorin I antibody 83250-2-RRDownload protocol
WB protocol for Ribophorin I antibody 83250-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...