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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPLP2 Polyclonal antibody

RPLP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16805-1-AP

Synonyms

D11S2243E, P2, ribosomal protein, large, P2, RPLP2, RPP2

Validation Data Gallery

Applications:
  • WB analysis using 16805-1-AP

    WB analysis using 16805-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16805-1-AP (RPLP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 16805-1-AP

    IHC staining of mouse kidney using 16805-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16805-1-AP (RPLP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPLP2 antibody included
  • IHC staining of mouse kidney using 16805-1-AP

    IHC staining of mouse kidney using 16805-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16805-1-AP (RPLP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPLP2 antibody included
  • IHC staining of human breast cancer using 16805-1-AP

    IHC staining of human breast cancer using 16805-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16805-1-AP (RPLP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPLP2 antibody included
  • IHC staining of human breast cancer using 16805-1-AP

    IHC staining of human breast cancer using 16805-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16805-1-AP (RPLP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPLP2 antibody included

Tested Applications

Positive WB detected inHL-60 cells, mouse liver tissue
Positive IHC detected inmouse kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RPLP2 Ready-To-Use IHC Kit. RPLP2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16805-1-AP targets RPLP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPLP2 fusion protein Ag10293 相同性解析による交差性が予測される生物種
Full Name ribosomal protein, large, P2
Calculated molecular weight 115 aa, 12 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC062314
Gene symbol RPLP2
Gene ID (NCBI) 6181
RRIDAB_2878318
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPLP2 antibody 16805-1-APDownload protocol
IHC protocol for RPLP2 antibody 16805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells

Authors - Chandreyee Datta
View Article