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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL9 Monoclonal antibody (68054-1-Ig)

    WB analysis using 68054-1-Ig (same clone as 68054-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68054-1-Ig (RPL9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IHC staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IHC staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IF Staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IF Staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), FUS/TLS antibody (11570-1-AP, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IF Staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

RPL9 Monoclonal antibody, PBS Only

RPL9 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 68054-1-PBS
Clone No.2A6B11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

2A6B11, 60S ribosomal protein L9, FLJ27456, Large ribosomal subunit protein uL6, OK/SW-cl.103

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68054-1-PBS targets RPL9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5391

Product name: Recombinant human RPL9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-192 aa of BC066318

Sequence: MKTILSNQTVDIPENVDITLKGRTVIVKGPRGTLRRDFNHINVELSLLGKKKKRLRVDKWWGNRKELATVRTICSHVQNMIKGVTLGFRYKMRSVYAHFPINVVIQENGSLVEIRNFLGEKYIRRVRMRPGVACSVSQAQKDELILEGNDIELVSNSAALIQQATTVKNKDIRKFLDGIYVSEKGTVQQADE

相同性解析による交差性が予測される生物種
Full Name ribosomal protein L9
Calculated molecular weight 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC066318
Gene Symbol RPL9
Gene ID (NCBI) 6133
RRIDAB_2918795
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP32969
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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