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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL7A Recombinant antibody (86270-4-RR)

    WB analysis using 86270-4-RR (same clone as 86270-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86270-4-RR (RPL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86270-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL7A Recombinant antibody (86270-4-RR)

    IF Staining of HepG2 using 86270-4-RR (same clone as 86270-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL7A antibody (86270-4-RR, Clone: 250886G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86270-4-PBS in a different storage buffer formulation.

RPL7A Recombinant monoclonal antibody, PBS Only

RPL7A Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86270-4-PBS
Clone No.250886G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

60S ribosomal protein L7a, Large ribosomal subunit protein eL8, PLA X polypeptide, PLA-X polypeptide, ribosomal protein L7a

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86270-4-PBS targets RPL7A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7543

Product name: Recombinant human RPL7A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-266 aa of BC005128

Sequence: MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG

相同性解析による交差性が予測される生物種
Full Name ribosomal protein L7a
Calculated molecular weight 266 aa, 30 kDa
Observed molecular weight32 kDa
GenBank accession numberBC005128
Gene Symbol RPL7A
Gene ID (NCBI) 6130
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP62424
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].

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