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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL6 Monoclonal antibody (67729-1-Ig)

    WB analysis using 67729-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67729-1-Ig (RPL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using RPL6 Monoclonal antibody (67729-1-Ig)

    IF Staining of L02 using 67729-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using RPL6 antibody (67729-1-Ig, Clone: 3F6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

RPL6 Monoclonal antibody

RPL6 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67729-1-Ig
Clone No.3F6F8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

3F6F8, 60S ribosomal protein L6, Large ribosomal subunit protein eL6, Neoplasm related protein C140, Neoplasm-related protein C140

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67729-1-Ig targets RPL6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6840

Product name: Recombinant human RPL6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-288 aa of BC004138

Sequence: MAGEKVEKPDTKEKKPEAKKVDAGGKVKKGNLKAKKPKKGKPHCSRNPVLVRGIGRYSRSAMYSRKAMYKRKYSAAKSKVEKKKKEKVLATVTKPVGGDKNGGTRVVKLRKMPRYYPTEDVPRKLLSHGKKPFSQHVRKLRASITPGTILIILTGRHRGKRVVFLKQLASGLLLVTGPLVLNRVPLRRTHQKFVIATSTKIDISNVKIPKHLTDAYFKKKKLRKPRHQEGEIFDTEKEKYEITEQRKIDQKAVDSQILPKIKAIPQLQGYLRSVFALTNGIYPHKLVF

相同性解析による交差性が予測される生物種
Full Name ribosomal protein L6
Calculated molecular weight 33 kDa
Observed molecular weight34 kDa
GenBank accession numberBC004138
Gene Symbol RPL6
Gene ID (NCBI) 6128
RRIDAB_2882915
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02878
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomes are large multiple protein complexes which is composed of more than 80 components and provide a protein-synthesizing machinery for all living cells. Ribosomal protein like 6 (RPL6, also called Taxreb107 for Tax responsive element binding protein 107), one of the ribosomal components, can bind with the Tax responsive element of human T cell leukemia virus (HTLV)-I in addition to be assembled to ribosomes [PMID:12031506]. Overexpressed in gastric cancer could promote cell growth and cell cycle progression at least through upregulating cyclin E expression[PMID:20171175].

Protocols

Product Specific Protocols
WB protocol for RPL6 antibody 67729-1-IgDownload protocol
IF protocol for RPL6 antibody 67729-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols