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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL5 Recombinant antibody (85348-1-RR)

    WB analysis using 85348-1-RR (same clone as 85348-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85348-1-RR (RPL5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85348-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 3T3-L1 cells using RPL5 Recombinant antibody (85348-1-RR)

    WB analysis of 3T3-L1 using 85348-1-RR (same clone as 85348-1-PBS)

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 85348-1-RR (RPL5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85348-1-PBS in a different storage buffer formulation.

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RPL5 Recombinant antibody, PBS Only

RPL5 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85348-1-PBS
Clone No.242678D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85348-1-PBS targets RPL5 in applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPL5 fusion protein Ag30837 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L5
Observed molecular weight 34 kDa
GenBank accession numberBC109370
Gene Symbol RPL5
Gene ID (NCBI) 6125
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46777
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RPL5 (Ribosomal Protein L5) is a crucial component of the 60S ribosomal subunit, playing a significant role in ribosome biogenesis and function. It is required for rRNA maturation and the formation of the 60S ribosomal subunits. Defects or mutations in RPL5 are associated with Diamond-Blackfan anemia (DBA), a congenital disorder characterized by red blood cell aplasia.(PMID: 28376382)