Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, PC-12 cells, RAW 264.7 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15185-1-AP targets RPL36A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7210 Product name: Recombinant human RPL36A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-106 aa of BC001781 Sequence: MVNVPKTRRTFCKKCGKHQPHKVTQYKKGKDSLYAQGKRRYDRKQSGYGGQTKPIFRKKAKTTKKIVLRLECVEPNCRSKRMLAIKRCKHFELGGDKKRKGQVIQF 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein L36a |
| Calculated molecular weight | 12 kDa |
| Observed molecular weight | 12-17 kDa |
| GenBank accession number | BC001781 |
| Gene Symbol | RPL36A |
| Gene ID (NCBI) | 6173 |
| RRID | AB_3669200 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P83881 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RPL36A antibody 15185-1-AP | Download protocol |
| WB protocol for RPL36A antibody 15185-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |