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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL29 Recombinant antibody (83377-1-RR)

    WB analysis using 83377-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83377-1-RR (RPL29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL29 Recombinant antibody (83377-1-RR)

    IF Staining of HepG2 using 83377-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL29 Recombinant antibody (83377-1-RR)

    IF Staining of HepG2 using 83377-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using RPL29 Recombinant antibody (83377-1-RR)

    FC experiment of HeLa using 83377-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPL29 Recombinant antibody (83377-1-RR, Clone:240062F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83377-1-RR

    Affinity and Kinetic Characterization of 83377-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83377-1-RR against Human RPL29 were performed. The affinity constant is 0.254 nM.

RPL29 Recombinant monoclonal antibody

RPL29 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83377-1-RR
Clone No.240062F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240062F9, 60S ribosomal protein L29, Cell surface heparin-binding protein HIP, HIP, HUMRPL29

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells, U-87 MG cells, C6 cells, NIH/3T3 cells,
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83377-1-RR targets RPL29 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34100

Product name: Recombinant human RPL29 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-97 aa of BC008926

Sequence: MAKSKNHTTHNQSRKWHRNGIKKPRSQRYESLKGVDPKFLRNMRFAKKHNKKGLKKMQANNAKAMSARAEAIKALVKPKEVKPKIPKGVSRKLDRLA

相同性解析による交差性が予測される生物種
Full Name ribosomal protein L29
Calculated molecular weight 159 aa, 18 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC008926
Gene Symbol RPL29
Gene ID (NCBI) 6159
RRIDAB_3671030
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP47914
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal protein L29 (Rpl29) is a component of the large (60S) ribosomal subunit which is abundantly expressed in all cell types, plays a regulatory role in translation efficiency and is essential for protein synthesis (PMID: 17195189). In contrast to adult tissues, RPL29 displays a much more widespread pattern of expression throughout embryonic development, and high levels of RPL29 are found in all developing organs. Several reports showed that RPL29 expression is up-regulated in human carcinomas and constitutes a candidate marker for abnormal growth when overexpressed(PMID: 9788632, 10383165).

Protocols

Product Specific Protocols
IF protocol for RPL29 antibody 83377-1-RRDownload protocol
WB protocol for RPL29 antibody 83377-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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