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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL29 Polyclonal antibody (15799-1-AP)

    WB analysis using 15799-1-AP (same clone as 15799-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15799-1-AP (RPL29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RPL29 Polyclonal antibody (15799-1-AP)

    WB analysis using 15799-1-AP (same clone as 15799-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15799-1-AP (RPL29 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPL29 Polyclonal antibody (15799-1-AP)

    IHC staining of mouse brain using 15799-1-AP (same clone as 15799-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15799-1-AP (RPL29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPL29 Polyclonal antibody (15799-1-AP)

    IHC staining of human breast cancer using 15799-1-AP (same clone as 15799-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RPL29 Polyclonal antibody (15799-1-AP)

    IHC staining of human liver cancer using 15799-1-AP (same clone as 15799-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPL29 Polyclonal antibody (15799-1-AP)

    IHC staining of mouse brain using 15799-1-AP (same clone as 15799-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15799-1-AP (RPL29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RPL29 Polyclonal antibody (15799-1-AP)

    IHC staining of human liver cancer using 15799-1-AP (same clone as 15799-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPL29 Polyclonal antibody (15799-1-AP)

    IHC staining of human breast cancer using 15799-1-AP (same clone as 15799-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL29 Polyclonal antibody (15799-1-AP)

    IF Staining of HepG2 using 15799-1-AP (same clone as 15799-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL29 antibody (15799-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

RPL29 Polyclonal antibody, PBS Only

RPL29 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15799-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15799-1-PBS targets RPL29 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8510

Product name: Recombinant human RPL29 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-159 aa of BC008926

Sequence: MAKSKNHTTHNQSRKWHRNGIKKPRSQRYESLKGVDPKFLRNMRFAKKHNKKGLKKMQANNAKAMSARAEAIKALVKPKEVKPKIPKGVSRKLDRLAYIAHPKLGKRARARIAKGLRLCRPKAKAKAKAKDQTKAQAAAPASVPAQAPKRTQAPTKASE

相同性解析による交差性が予測される生物種
Full Name ribosomal protein L29
Calculated molecular weight 159 aa, 18 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC008926
Gene Symbol RPL29
Gene ID (NCBI) 6159
RRIDAB_2878187
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP47914
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ribosomal protein L29 (Rpl29) is a component of the large (60S) ribosomal subunit which is abundantly expressed in all cell types, plays a regulatory role in translation efficiency and is essential for protein synthesis (PMID: 17195189). In contrast to adult tissues, RPL29 displays a much more widespread pattern of expression throughout embryonic development, and high levels of RPL29 are found in all developing organs. Several reports showed that RPL29 expression is up-regulated in human carcinomas and constitutes a candidate marker for abnormal growth when overexpressed(PMID: 9788632, 10383165).The antibody can detect the band around 22kDa.

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