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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using RPL27A Polyclonal antibody (16002-1-AP)

    WB analysis of A549 using 16002-1-AP (same clone as 16002-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 16002-1-AP (RPL27A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 16002-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using RPL27A Polyclonal antibody (16002-1-AP)

    WB analysis of A549 using 16002-1-AP (same clone as 16002-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 16002-1-AP (RPL27A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 16002-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using RPL27A Polyclonal antibody (16002-1-AP)

    IHC staining of mouse stomach using 16002-1-AP (same clone as 16002-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16002-1-AP (RPL27A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16002-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using RPL27A Polyclonal antibody (16002-1-AP)

    IHC staining of mouse stomach using 16002-1-AP (same clone as 16002-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16002-1-AP (RPL27A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16002-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RPL27A Polyclonal antibody (16002-1-AP)

    IF Staining of A549 using 16002-1-AP (same clone as 16002-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RPL27A antibody (16002-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16002-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RPL27A Polyclonal antibody, PBS Only

RPL27A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16002-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

60S ribosomal protein L27a, Large ribosomal subunit protein uL15, ribosomal protein L27a

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16002-1-PBS targets RPL27A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8596

Product name: Recombinant human RPL27A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-148 aa of BC005326

Sequence: MPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA

相同性解析による交差性が予測される生物種
Full Name ribosomal protein L27a
Calculated molecular weight 148 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC005326
Gene Symbol RPL27A
Gene ID (NCBI) 6157
RRIDAB_2878206
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP46776
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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