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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL26 Recombinant antibody (85775-1-RR)

    WB analysis using 85775-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85775-1-RR (RPL26 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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RPL26 Recombinant antibody

RPL26 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85775-1-RR
Clone No.243015D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, ELISA

Synonyms

60S ribosomal protein L26, Large ribosomal subunit protein uL24, ribosomal protein L26

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, COS-7 cells, NIH/3T3 cells, PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85775-1-RR targets RPL26 in WB, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPL26 fusion protein Ag11821 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L26
Calculated molecular weight 145 aa, 17 kDa
Observed molecular weight 17-22 kDa
GenBank accession numberBC071664
Gene Symbol RPL26
Gene ID (NCBI) 6154
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61254
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPL26, or Ribosomal Protein L26, is a crucial component of the ribosome, the cellular machinery responsible for protein synthesis. It belongs to the large subunit of the ribosome and plays a significant role in ribosome biogenesis and function. RPL26 is involved in the translation process, where it helps decode mRNA into proteins, ensuring proper cellular function and development. RPL26 mutations have been implicated in Diamond-Blackfan anemia (DBA), a rare congenital disorder characterized by erythroid hypoplasia. (PMID: 30355487; 24675553)

Protocols

Product Specific Protocols
WB protocol for RPL26 antibody 85775-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols