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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL23 Recombinant antibody (86520-1-RR)

    WB analysis using 86520-1-RR (same clone as 86520-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86520-1-RR (RPL23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86520-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPL23 Recombinant antibody (86520-1-RR)

    IF Staining of HeLa using 86520-1-RR (same clone as 86520-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPL23 antibody (86520-1-RR, Clone: 251372C7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86520-1-PBS in a different storage buffer formulation.

RPL23 Recombinant monoclonal antibody, PBS Only

RPL23 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86520-1-PBS
Clone No.251372C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Large ribosomal subunit protein uL14, ribosomal protein L23, rpL17

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86520-1-PBS targets RPL23 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9120

Product name: Recombinant human RPL23 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-140 aa of BC010114

Sequence: MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA

相同性解析による交差性が予測される生物種
Full Name ribosomal protein L23
Calculated molecular weight 140 aa, 15 kDa
Observed molecular weight15 kDa
GenBank accession numberBC010114
Gene Symbol RPL23
Gene ID (NCBI) 9349
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP62829
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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