Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of A431 using 25002-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 25002-1-AP (RPL22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 25002-1-AP
WB result of RPL22 antibody (25002-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPL22 transfected A431 cells.
WB analysis of HepG2 using 25002-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 25002-1-AP (RPL22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 25002-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 25002-1-AP (RPL22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25002-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25002-1-AP (RPL22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 25002-1-AP
IP result of anti-RPL22 (IP:25002-1-AP, 4ug; Detection:25002-1-AP 1:500) with A431 cells lysate 1920 ug.
IF Staining of HeLa using 25002-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPL22 antibody (25002-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

RPL22 Polyclonal antibody

RPL22 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 25002-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

60S ribosomal protein L22, EAP, EBER associated protein, EBER-associated protein, eL22

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, HepG2 cells, HeLa cells, Jurkat cells
Positive IP detected in	A431 cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 8 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

25002-1-AP targets RPL22 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21851 Product name: Recombinant human RPL22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-128 aa of BC058887 Sequence: MAPVKKLVVKGGKKKKQVLKFTLDCTHPVEDGIMDAANFEQFLQERIKVNGKAGNLGGGVVTIERSKSKITVTSEVPFSKRYLKYLTKKYLKKNNLRDWLRVVANSKESYELRYFQINQDEEEEEDED 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein L22
Calculated molecular weight	15 kDa
Observed molecular weight	15-18 kDa
GenBank accession number	BC058887
Gene Symbol	RPL22
Gene ID (NCBI)	6146
RRID	AB_2879841
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35268
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for RPL22 antibody 25002-1-AP	Download protocol
IP protocol for RPL22 antibody 25002-1-AP	Download protocol
WB protocol for RPL22 antibody 25002-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC. Authors - Martin Gamerdinger View Article
human,mouse	WB,IP,IF	Acta Neuropathol Commun Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease Authors - Suman Rimal View Article
human	WB	Cell Death Discov N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness Authors - Hongliang Dong View Article
human	WB,IF	Carcinogenesis The Molecular Landscape of Synchronous Colorectal Cancer Reveals Genetic Heterogeneity. Authors - Xiangfeng Wang View Article
human	WB	Mol Neurobiol Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation Authors - Ruicheng Yang View Article
human	IF	Cell Rep The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress Authors - Jennifer Jansen KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A431 using 25002-1-AP

WB analysis of A431 using 25002-1-AP

WB analysis of HepG2 using 25002-1-AP

WB analysis of Jurkat using 25002-1-AP

WB analysis of HeLa using 25002-1-AP

IP experiment of A431 using 25002-1-AP

IF Staining of HeLa using 25002-1-AP

RPL22 Polyclonal antibody

RPL22 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC.

Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease

N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness

The Molecular Landscape of Synchronous Colorectal Cancer Reveals Genetic Heterogeneity.

Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation

The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress