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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of HepG2 using 14653-1-AP (same clone as 14653-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of HeLa using 14653-1-AP (same clone as 14653-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of human brain using 14653-1-AP (same clone as 14653-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of mouse lung using 14653-1-AP (same clone as 14653-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of PC-3 using 14653-1-AP (same clone as 14653-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RPL18A Polyclonal antibody (14653-1-AP)

    IHC staining of human prostate cancer using 14653-1-AP (same clone as 14653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RPL18A Polyclonal antibody (14653-1-AP)

    IHC staining of human prostate cancer using 14653-1-AP (same clone as 14653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL18A Polyclonal antibody (14653-1-AP)

    IF Staining of HepG2 using 14653-1-AP (same clone as 14653-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL18A antibody (14653-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL18A Polyclonal antibody (14653-1-AP)

    IF Staining of HepG2 using 14653-1-AP (same clone as 14653-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using RPL18A antibody 14653-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

RPL18A Polyclonal antibody, PBS Only

RPL18A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14653-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14653-1-PBS targets RPL18A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6251

Product name: Recombinant human RPL18A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-176 aa of BC066319

Sequence: MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF

相同性解析による交差性が予測される生物種
Full Name ribosomal protein L18a
Calculated molecular weight 21 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC066319
Gene Symbol RPL18A
Gene ID (NCBI) 6142
RRIDAB_2181576
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02543
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RPL18A, also named as 60S ribosomal protein L18a(Ribosomal Protein L18a), located on 19p13.11, is a protein coding gene. RPL18A is a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). Diseases such as Hepatitis C Virus and Omsk Hemorrhagic Fever are associated with RPL18A. The molecular weight of RPL18A is 23 kDa.