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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL18 Polyclonal antibody

RPL18 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17029-1-AP

Synonyms

60S ribosomal protein L18, ribosomal protein L18, RPL18

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 17029-1-AP

    WB analysis of HeLa using 17029-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 17029-1-AP

    WB analysis of HeLa using 17029-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 17029-1-AP

    WB analysis of HepG2 using 17029-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 17029-1-AP

    WB analysis of HEK-293 using 17029-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 17029-1-AP

    WB analysis of HepG2 using 17029-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17029-1-AP is RPL18 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL18 fusion protein Ag10474 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L18
Calculated molecular weight 188 aa, 22 kDa
Observed molecular weight 22-26 kDa
GenBank accession numberBC009708
Gene symbol RPL18
Gene ID (NCBI) 6141
RRIDAB_2878340
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL18 antibody 17029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Virus Res

Up-regulated 60S ribosomal protein L18 in PEDV N protein-induced S-phase arrested host cells promotes viral replication

Authors - Qinghe Zhu
  • KD Validated
View Article
humanWB

iScience

TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment

Authors - Magdalena Wolczyk
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Katie (Verified Customer) (05-23-2022)

Gives appropriate sized band in western blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: T cells - mouse