Validation Data Gallery

Filter: AllWB

WB analysis of HeLa using 17029-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17029-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17029-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17029-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17029-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RPL18 Polyclonal antibody

RPL18 Polyclonal Antibody for WB, ELISA

Cat No. 17029-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

60S ribosomal protein L18, ribosomal protein L18, RPL18

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

HeLa cells, HEK-293 cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below

Product Information

17029-1-AP targets RPL18 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPL18 fusion protein Ag10474 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein L18
Calculated molecular weight	188 aa, 22 kDa
Observed molecular weight	22-26 kDa
GenBank accession number	BC009708
Gene Symbol	RPL18
Gene ID (NCBI)	6141
RRID	AB_2878340
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q07020
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL18 antibody 17029-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
pig	WB	Virus Res Up-regulated 60S ribosomal protein L18 in PEDV N protein-induced S-phase arrested host cells promotes viral replication Authors - Qinghe Zhu KD Validated View Article
human	WB	iScience TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment Authors - Magdalena Wolczyk View Article

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