Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, HeLa cells, SKOV-3 cells, mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16222-1-AP targets RPL10L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9275 Product name: Recombinant human RPL10L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC014310 Sequence: MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLGGHMVSDEYEQLSSEALEAARICANKYMVKSCGRDGFHMRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNEEHVIEALRRAKFKFPGRQKIHISKKWGFTKFNADEFEDMVAKKCLIPDGCGVKYVPSHGPLDKWRVLHS 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein L10-like |
| Calculated molecular weight | 214 aa, 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC014310 |
| Gene Symbol | RPL10L |
| Gene ID (NCBI) | 140801 |
| RRID | AB_3085482 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96L21 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPL10L (Ribosomal Protein L10 Like), is a testis-specific component of the ribosome, is responsible for regulating the biosynthesis and folding of a subset of male germ-cell-specific proteins that are essential for the formation of sperm(Uniprot). RPL10L plays an essential role during meiosis of spermatogenesis by compensating for its X-linked parental paralog, RPL10, during and after meiotic sex chromosome inactivation(MSCI) (PMID: 28502657).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL10L antibody 16222-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |