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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPIA Polyclonal antibody

RPIA Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 13010-1-AP

Synonyms

Phosphoriboisomerase, Ribose 5 phosphate isomerase, ribose 5 phosphate isomerase A, RPI, RPIA

Validation Data Gallery

Applications:
  • WB analysis using 13010-1-AP

    WB analysis using 13010-1-AP

    mouse spleen tissues were subjected to SDS PAGE followed by western blot with 13010-1-AP (RPIA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 13010-1-AP

    IF Staining of HepG2 using 13010-1-AP

    Immunofluorescent analysis of HepG2 cells, using RPIA antibody 13010-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse spleen tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13010-1-AP targets RPIA in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPIA fusion protein Ag3699 相同性解析による交差性が予測される生物種
Full Name ribose 5-phosphate isomerase A
Calculated molecular weight 311 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC015529
Gene symbol RPIA
Gene ID (NCBI) 22934
RRIDAB_10638301
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPIA(Ribose-5-phosphate isomerase) is also named as RPI and belongs to the ribose 5-phosphate isomerase family. It is involved in pentose-phosphate shunt, non-oxidative branch. It is an enzyme in the pentose-phosphate pathway, appears to be determined by a single structural locus and is probably a dimer(PMID:7396409).

Protocols

Product Specific Protocols
WB protocol for RPIA antibody 13010-1-APDownload protocol
IF protocol for RPIA antibody 13010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols