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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using RPH3A Polyclonal antibody (11396-1-AP)

    WB analysis of COLO 320 using 11396-1-AP (same clone as 11396-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 11396-1-AP (RPH3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RPH3A Polyclonal antibody (11396-1-AP)

    WB analysis of human brain using 11396-1-AP (same clone as 11396-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11396-1-AP (RPH3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPH3A Polyclonal antibody (11396-1-AP)

    IHC staining of mouse brain using 11396-1-AP (same clone as 11396-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11396-1-AP (RPH3A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPH3A Polyclonal antibody (11396-1-AP)

    IHC staining of mouse brain using 11396-1-AP (same clone as 11396-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11396-1-AP (RPH3A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using RPH3A Polyclonal antibody (11396-1-AP)

    IF Staining of SH-SY5Y using 11396-1-AP (same clone as 11396-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using RPH3A antibody (11396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

RPH3A Polyclonal antibody, PBS Only

RPH3A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11396-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Rabphilin 3A, Rabphilin, KIAA0985, Exophilin-1, Exophilin1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11396-1-PBS targets RPH3A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1956

Product name: Recombinant human RPH3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC017259

Sequence: MTDTVFSNSSNRWMYPSDRPLQSKLQAGWSVHPGGQPDRQRKQEELTDEEKEIINRVIARAEKMEEMEQERIGRLVDRLENMRKNVAGDGVNRCILCGEQLGMLGSACVVCEDCKKNVCTKCGVETNNRLHSVWLCKICIEQREVWKRSGAWFFKGFPKQVLPQPMPIKKTKPQQPVSEPAAPEQPAPEPKHPARAPARGDSEDRRGPGQKTGPDPASAPGRGNYGPPVRRASEARMSSSSRDSESWDHSGGAGDSSRSPAGLRRANSVQASRPAPGSVQSPAPPQPGQPGTPGGSRPGP

相同性解析による交差性が予測される生物種
Full Name rabphilin 3A homolog (mouse)
Calculated molecular weight 690 aa, 76 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC017259
Gene Symbol RPH3A
Gene ID (NCBI) 22895
RRIDAB_2181145
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2J0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAB3A is a small G protein that is thought to act in exocytosis of neurotransmitters and hormones in synaptic neurotransmission and cell-cell communication. RPH3A (RPH3A), a RAB3A effector, is a neuronal C2 domain tandem containing protein that is involved in the neuronal transmitter process. Huntington's disease (HD) is a hereditary neurodegenerative disorder characterized by progressive motor disturbances, cognitive defects and dementia. Level of rabphilin 3A is substantially decreased in synapses of most brain regions in R6/1 transgenic mouse model of HD suggesting a role of RPH3A in impaired synaptic transmission.

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