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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPAIN Monoclonal antibody (68546-1-Ig)

    WB analysis using 68546-1-Ig (same clone as 68546-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68546-1-Ig (RPAIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68546-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RPAIN Monoclonal antibody (68546-1-Ig)

    WB analysis using 68546-1-Ig (same clone as 68546-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68546-1-Ig (RPAIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68546-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A2780 cells using RPAIN Monoclonal antibody (68546-1-Ig)

    WB analysis of A2780 using 68546-1-Ig (same clone as 68546-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 68546-1-Ig (RPAIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68546-1-PBS in a different storage buffer formulation.

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RPAIN Monoclonal antibody, PBS Only

RPAIN Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68546-1-PBS
Clone No.2D8E3

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

HRIP, RIP, RPA interacting protein, RPAIN

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68546-1-PBS targets RPAIN in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPAIN fusion protein Ag7844 相同性解析による交差性が予測される生物種
Full Name RPA interacting protein
Calculated molecular weight 25 kDa
Observed molecular weight25 kDa
GenBank accession numberBC004451
Gene Symbol RPAIN
Gene ID (NCBI) 84268
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86UA6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Replication protein A (RPA) is a eukaryotic single-stranded DNA binding protein that is essential for DNA replication, repair, and recombination, and human RPA interacting protein α (hRIPα) is the nuclear transporter of RPA. RPAIN was originally identified as a nuclear transporter of RPA in Xenopus (PMID: 23010595). The human RIPα gene encodes several splice isoforms, of which hRIPα and hRIPβ are the major translation products in vivo.hRIPα transports RPA into the nucleus by shuttling between the cytoplasm and nucleus while hRIPβ is localized in promyelocytic leukemia (PML) nuclear bodies.