• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A2780 cells using RPAIN Polyclonal antibody (15220-1-AP)

    WB analysis of A2780 using 15220-1-AP (same clone as 15220-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using RPAIN Polyclonal antibody (15220-1-AP)

    WB analysis of A375 using 15220-1-AP (same clone as 15220-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using RPAIN Polyclonal antibody (15220-1-AP)

    WB analysis of mouse ovary using 15220-1-AP (same clone as 15220-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A375 cells using RPAIN Polyclonal antibody (15220-1-AP)

    IP experiment of A375 using 15220-1-AP (same clone as 15220-1-PBS)

    IP result of anti-RPAIN (IP:15220-1-AP, 4ug; Detection:15220-1-AP 1:300) with A375 cells lysate 2200 ug. This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RPAIN Polyclonal antibody (15220-1-AP)

    IHC staining of human ovary tumor using 15220-1-AP (same clone as 15220-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15220-1-AP (RPAIN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RPAIN Polyclonal antibody (15220-1-AP)

    IHC staining of human ovary tumor using 15220-1-AP (same clone as 15220-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15220-1-AP (RPAIN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

RPAIN Polyclonal antibody, PBS Only

RPAIN Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15220-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15220-1-PBS targets RPAIN in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7392

Product name: Recombinant human RPAIN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-145 aa of BC004451

Sequence: MAESLRSPRRSLYKLVGSPPWKEAFRQRCLERMRNSRDRLLNRYRQAGSSGPGNSQNSFLVQEVMEEEWNALQSVENCPEDLAQLEELIDMAVLEEIQQELINQEQSIISEYEKSLQFDEKCLSIMLAEWEANPLICPVCTNLLS

相同性解析による交差性が予測される生物種
Full Name RPA interacting protein
Calculated molecular weight 25 kDa
Observed molecular weight 30-45 kDa
GenBank accession numberBC004451
Gene Symbol RPAIN
Gene ID (NCBI) 84268
RRIDAB_2180976
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UA6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PAIN, also named as RIP, mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. RPAIN has many isoforms with MW 25-27 kDa, 12 kDa and 16-19 kDa. The publication with PMID: 16135809 reported the MW 30 and 45 kDa.

||
New chat

Able™

正在加载，请稍候...