×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RPA2 Recombinant monoclonal antibody (87254-1-RR)

    WB analysis using 87254-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87254-1-RR (RPA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RPA2 Recombinant monoclonal antibody (87254-1-RR)

    IF Staining of A431 using 87254-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (87254-1-RR, Clone: 252472D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity of 87254-1-RR

    Affinity of 87254-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87254-1-RR against Human RPA were performed. The affinity constant is 1.22 nM.

RPA2 Recombinant monoclonal antibody

RPA2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87254-1-RR
Clone No.252472D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF-A protein 2, RP A p32

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat testis tissue, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, THP-1 cells, mouse testis tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87254-1-RR targets RPA2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0690

Product name: Recombinant human RPA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 80-270 aa of BC001630

Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

相同性解析による交差性が予測される生物種
Full Name replication protein A2, 32kDa
Calculated molecular weight 29 kDa
Observed molecular weight32 kDa
GenBank accession numberBC001630
Gene Symbol RPA2
Gene ID (NCBI) 6118
RRIDAB_3745434
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP15927
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Tr residues that are phosphorylated in sequential order in response to genotoxic stress.

Protocols

Product Specific Protocols
IF protocol for RPA2 antibody 87254-1-RRDownload protocol
WB protocol for RPA2 antibody 87254-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...