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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis using 67999-1-Ig (same clone as 67999-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis of pig spleen using 67999-1-Ig (same clone as 67999-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis of rat testis using 67999-1-Ig (same clone as 67999-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis of mouse testis using 67999-1-Ig (same clone as 67999-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit spleen tissue using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis of rabbit spleen using 67999-1-Ig (same clone as 67999-1-PBS)

    rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RPA2 Monoclonal antibody (67999-1-Ig)

    IF Staining of A431 using 67999-1-Ig (same clone as 67999-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (67999-1-Ig, Clone: 1B9H11 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

RPA2 Monoclonal antibody, PBS Only

RPA2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67999-1-PBS
Clone No.1B9H11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67999-1-PBS targets RPA2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24783

Product name: Recombinant human RPA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 80-270 aa of BC001630

Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

相同性解析による交差性が予測される生物種
Full Name replication protein A2, 32kDa
Calculated molecular weight 29 kDa
Observed molecular weight30 kDa
GenBank accession numberBC001630
Gene Symbol RPA2
Gene ID (NCBI) 6118
RRIDAB_2918747
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP15927
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.