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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RPA1 Monoclonal antibody

RPA1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G3F11

Cat no : CL488-67973

Synonyms

HSSB, MST075, REPA1, Replication factor A protein 1, replication protein A1, 70kDa, RF A, RF A protein 1, RP A, RP A p70, RPA1, RPA70

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-67973

    FC experiment of HeLa using CL488-67973

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RPA1 (CL488-67973, Clone:1G3F11) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67973-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67973 targets RPA1 in FC (Intra) applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPA1 fusion protein Ag28239 相同性解析による交差性が予測される生物種
Full Name replication protein A1, 70kDa
Calculated molecular weight 616 aa, 68 kDa
Observed molecular weight68 kDa
GenBank accession numberBC018126
Gene symbol RPA1
Gene ID (NCBI) 6117
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 RPA1 antibody CL488-67973Download protocol
Standard Protocols
Click here to view our Standard Protocols