Validation Data Gallery
Tested Applications
| Positive WB detected in | A-204 cells, MG-63 cells, SH-SY5Y cells, SK-N-SH cells, SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
34185-1-AP targets RP11-45B20.2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag41415 Product name: Recombinant human RP11-45B20.2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 197-333 aa of BC137004 Sequence: LVLPKSAFTVGLTVLSKFPSSDVPIKFDKILYNEFNHYDTAVGKFTCHIAGVYYFTYHITVFSRNVQVSLVKNGVKILHTRDAYVSSEDQASGSIVLQLKLGDEMWLQVTGGERFNGLFADEDDDTTFTGFLLFSSQ 相同性解析による交差性が予測される生物種 |
| Full Name | collagen triple helix repeat-containing |
| Observed molecular weight | 34-37 kDa |
| GenBank accession number | BC137004 |
| Gene Symbol | RP11-45B20.2 |
| Gene ID (NCBI) | 387911 |
| RRID | AB_3743168 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | B2RNN3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RP11-45B20.2(C1QTNF9B/CTRP9B) is a secreted protein with triple helix structure of collagen and C1q domain, belonging to CTRP family. It may participate in metabolic regulation by activating AMPK/AKT/MAPK signaling pathway, and it may be used as a potential prognostic marker in many cancers. The expression of this protein in retina also suggests that it may be related to eye diseases (such as high myopia). Its observed molecular weight is 34-37 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RP11-45B20.2 antibody 34185-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |