Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 23 publications below |
| IHC | See 6 publications below |
| IF | See 1 publications below |
| ChIP | See 2 publications below |
Product Information
13205-1-AP targets RORC in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3961 Product name: Recombinant human RORC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 165-518 aa of BC031554 Sequence: DLPEASACPPGLLKASGSGPSYSNNLAKAGLNGASCHLEYSPERGKAEGRESFYSTGSQLTPDRCGLRFEEHRHPGLGELGQGPDSYGSPSFRSTPEAPYASLTEIEHLVQSVCKSYRETCQLRLEDLLRQRSNIFSREEVTGYQRKSMWEMWERCAHHLTEAIQYVVEFAKRLSGFMELCQNDQIVLLKAGAMEVVLVRMCRAYNADNRTVFFEGKYGGMELFRALGCSELISSIFDFSHSLSALHFSEDEIALYTALVLINAHRPGLQEKRKVEQLQYNLELAFHHHLCKTHRQSILAKLPPKGKLRSLCSQHVERLQIFQHLHPIVVQAAFPPLYKELFSTETESPVGLSK 相同性解析による交差性が予測される生物種 |
| Full Name | RAR-related orphan receptor C |
| Calculated molecular weight | 518 aa, 58 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | BC031554 |
| Gene Symbol | RORC |
| Gene ID (NCBI) | 6097 |
| RRID | AB_2180306 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51449 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The Nuclear receptors retinoic acid receptor-related orphan receptor gamma (RORγ or RORc, also known as NR1F3) was the third and most recent ROR group member to be discovered, following the prior disclosures of RORα (RORa or NR1F1) and RORβ (RORb or NR1F2) (PMID:24502334). Studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2.RORC has 2 isoforms with the MW of 56 kDa and 58 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RORC antibody 13205-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Immunity A. faecalis induces intestinal T helper 17 cells by promoting E3 ubiquitin ligase Trim21-mediated E3 ubiquitin ligase Fbxw7 degradation | ||
Cancer Res Retinoic acid-related orphan receptor C regulates proliferation, glycolysis, and chemoresistance via the PD-L1/ITGB6/STAT3 signaling axis in bladder cancer. | ||
Phytomedicine Salidroside alleviates bone cancer pain by inhibiting Th17/Treg imbalance through the AMPK/SIRT1 pathway | ||
Phytomedicine Qing-Chang-Hua-Shi granule ameliorates DSS-induced colitis by activating NLRP6 signaling and regulating Th17/Treg balance | ||
Phytomedicine Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa | ||
Oncogene Targeting the cholesterol-RORα/γ axis inhibits colorectal cancer progression through degrading c-myc |