Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12565-1-AP targets ROPN1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3178 Product name: Recombinant human ROPN1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-230 aa of BC014607 Sequence: MPLPDTMFCAQQIHIPPELPDILKQFTKAAIRTQPADVLRWSAGYFSALSRGDPLPVKDRMEMPTATQKTDTGLTQGLLKVLHKQCHHKRYVELTDLEQKWKNLCLPKEKFKALLQLDPCENKIKWINFLALGCSMLGGSLNTALKHLCEILTDDREGGPARIPFKTFSYVYRYLARLDSDVSPLETESYLASLKENIDARKNGMIGLSDFFFPKRKLLESIENSEDVGH 相同性解析による交差性が予測される生物種 |
| Full Name | ropporin 1-like |
| Calculated molecular weight | 230 aa, 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC014607 |
| Gene Symbol | ROPN1L |
| Gene ID (NCBI) | 83853 |
| RRID | AB_10638785 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96C74 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ROPN1L (Rhophilin Associated Tail Protein 1 Like) is a protein-coding gene that is a member of the ropporin family. ROPN1L plays a role in sperm function, as mutations in ROPN1L can cause defects in the fibrous sheath integrity, sperm motility, and PKA-dependent signaling processes, leading to male infertility.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ROPN1L antibody 12565-1-AP | Download protocol |
| WB protocol for ROPN1L antibody 12565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
