Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, human kidney tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
17047-1-AP targets ROGDI in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat, mouse, Drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10688 Product name: Recombinant human ROGDI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-287 aa of BC012901 Sequence: MATVMAATAAERAVLEEEFRWLLHDEVHAVLKQLQDILKEASLRFTLPGSGTEGPAKQENFILGSCGTDQVKGVLTLQGDALSQADVNLKMPRNNQLLHFAFREDKQWKLQQIQDARNHVSQAIYLLTSRDQSYQFKTGAEVLKLMDAVMLQLTRARNRLTTPATLTLPEIAASGLTRMFAPALPSDLLVNVYINLNKLCLTVYQLHALQPNSTKNFRPAGGAVLHSPGAMFEWGSQRLEVSHVHKVECVIPWLNDALVYFTVSLQLCQQLKDKISVFSSYWSYRPF 相同性解析による交差性が予測される生物種 |
| Full Name | rogdi homolog (Drosophila) |
| Calculated molecular weight | 287 aa, 32 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC012901 |
| Gene Symbol | ROGDI |
| Gene ID (NCBI) | 79641 |
| RRID | AB_2182328 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZN7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ROGDI encoded by the ROGDI gene, which encodes a leucine-zipper protein with high expression in the human spinal cord and brain, acts as a positive regulator of cell proliferation. Defections of ROGDI are the cause of Kohlschuetter-Toenz syndrome (KTZS)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ROGDI antibody 17047-1-AP | Download protocol |
| IP protocol for ROGDI antibody 17047-1-AP | Download protocol |
| WB protocol for ROGDI antibody 17047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Hum Genet A nonsense mutation in the human homolog of Drosophila rogdi causes Kohlschutter-Tonz syndrome. | ||
Sci Rep The Kohlschütter-Tönz syndrome associated gene Rogdi encodes a novel presynaptic protein. | ||
Cancer Biol Ther Downregulation of a novel human gene, ROGDI, increases radiosensitivity in cervical cancer cells.
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